Table 1. Multiplex Bins of Polymorphic SSR Primers for NM24016 x 3-79
|
Bin |
[Mg2+] (mM) |
Dilution of PCR products |
Blue (6FAM) |
Green (HEX) |
Yellow (NED) |
||||
|---|---|---|---|---|---|---|---|---|---|
|
Locus |
Size(bp) Tm1 / 3-79 |
Locus |
Size(bp) Tm1 / 3-79 |
Locus |
Size(bp) Tm1 / 3-79 |
||||
|
1 |
3 |
1:12 |
MP2572 |
254.5 / 238.5 |
MP3442 |
132.0 / 144.0 |
MP3556 |
138.6 / 134.6 |
|
|
|
|
MP3084 |
163.4 / 160.6 |
MP3147 |
164.9 / 155.3 |
||||
|
|
|
MP2634 |
254.1 / 264.0 |
MP3563 |
231.0 / 247.0 |
||||
|
2A |
3 |
1:12 |
MP1672 |
106.4 / 100.8 |
MP1414-1 |
138.7 / 128.2 |
MP1064 |
145.0 / 153.2 |
|
|
MP1317-1 |
181.9 / 174.2 |
MP1414-2 |
161.6 / 165.4 |
MP3065 |
191.7 / 183.5 |
||||
|
MP1317-2 |
201.2 / 209.3 |
MP1053 |
193.5 / 177.2 |
|
|
||||
|
2B |
3 |
1:3 |
MP2960 |
151.9 / 185.7 |
MP1679 |
164.1 / 191.4 |
MP169 |
198.5 / 214.0 |
|
|
3 |
2.5 |
1:10 |
MP3279 |
125.2 / 108.1 |
MP3482 |
140.6 / 146.4 |
MP840 |
164.6 / 154.6 |
|
|
|
|
MP3599-1 |
193.7 / 179.3 |
MP1350 |
196.7 / 206.8 |
||||
|
|
|
MP3599-2 |
216.1 / 212.4 |
|
|
||||
|
4A |
2.5 |
1:10 |
MP786 |
120.1 / 107.2 |
|
|
MP3816 |
201.6 / 186.0 |
|
|
|
|
|
|
MP3971 |
145.7 / 120.9 |
||||
|
4B |
2.5 |
1:10 |
MP1721 |
187.9 / 176.1 |
MP3255 |
232.0 / 209.1 |
|
|
|
|
5 |
2.5 |
1:10 |
MP3449 |
150.3 / 134.6 |
CLM60 |
124.7 / 119.0 |
CLM63 |
137.2 / 125.0 |
|
|
MP3627 |
183.8 / 175.7 |
|
|
MP1665 |
161.5 / 149.0 |
||||
|
|
|
|
|
MP3441 |
212.0 / 204.0 |
||||
|
6 |
3.25 |
1:5 |
MP3090 |
214.7 / 226.9 |
MP3792 |
233.6 / 214.8 |
MP2895 |
209.8 / 229.9 |
|
|
MP256 |
208.2 / 188.3 |
MP686-1 |
158.3 / 143.9 |
MP252 |
178.9 / 169.1 |
||||
|
|
|
MP686-2 |
185.1 / 189.0 |
|
|
||||
|
7 |
2.5 |
1:10 |
MP3034 |
156.6 / 174.2 |
MP3408-1 |
132.5 / 136.4 |
MP3383 |
191.3 / 177.1 |
|
|
|
|
MP3408-2 |
145.2 / 172.7 |
|
|
||||
|
8 |
2.5 |
1:10 |
MP3902 |
194.4 / 174.2 |
MP3895 |
186.0 / 197.9 |
|
|
|
|
9 |
2.5 |
1:30 |
|
|
MP3955 |
170.4 / 194.7 |
MP3649 |
193.2 / 193.2 |
|
|
10 |
3.25 |
1:10 |
|
|
MP3103 |
190.4 / 204.3 |
MP3995 |
198.1 / 188.8 |
|
|
11 |
2.5 |
1:8 |
CLM66-1 |
131.6 / 127.8 |
CLM43-1 |
100.5 / 102.5 |
MP2544 |
219.0 / 206.5 |
|
|
CLM66-2 |
139.8 / 161.4 |
CLM43-2 |
108.3 / 112.3 |
MP3479 |
246.7 / 254.4 |
||||
|
MP1440-1 |
239.6 / 243.4 |
MP3452 |
192.3 / 198.1 |
|
|
||||
|
MP1440-2 |
260.4 / 269.1 |
MP1059 |
217.9 / 230.1 |
|
|
||||
|
MP2590 |
189.6 / 205.6 |
|
|
|
|
||||
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